Source of founding: National Science Centre, SONATA Program, V edition
Duration: March 2014- March 2017
Project No.: 2013/09/D/ST8/04009
Project menager/ principal investigator: Dorota Kołbuk, Ph.D
Participants: Piotr Denis, M.Sc., Monika Bill, Maryla Moczulska
The aim of proposed research is to investigate polymers substrate supermolecular structure effect, mainly crystallinity, on the cells' activity and proliferation during in-vitro studies. The specific goal of the project is analysis of the degree of crystallinity in one- and two-componet polymers\foils in terms of cellular response. Determination of the mechanisms deciding about the increase of bioactivity of the material with an addition of gelatin is a supplementary aim.
The research project concerns the matter of polymer crystallinity influence on cells morphology, activity and proliferation. Commonly used methods forming three-dimensional scaffolds do not take into account crystallinity optimisation. It is therefore very important to know the influence of different crystallinity on cells response, without changing others parameters. To achieve this, it is necessary to analyse more in detail substrate based on one- and two-component foils, formed from the melt and solution. Degree of crystallinity differentiation is possible as an effect of investigation of fundamental relationship between molecular weight of polymer, speed of cooling (formation from melt); type of solvent and amount of biopolymer (formation from solutions).
Significance of the project for science/ Motivation
It is expected that an appropriate forming methodology render the optimal properties of one- and two-componet foils for efficient growth, activity and cells proliferation during the in-vitro study. It is anticipated that the proposed and implemented research will expand scientific knowledge about the crystallinity effect on the cell substrates functionality and contribute to the more complete literature description of the efficient degree of crystallinity control of the substrates formed by the other methods.